Current holders Blackpool start their defence of the Trophy away to Conference side York City, with last season's beaten finalists Southend facing Colchester.
The full draw:
Southern Section...
Colchester United v Southend United
Cheltenham Town v Dagenham & Redbridge
Boston United v Cambridge United
Walsall v Rushden & Diamonds
Oxford United v Exeter City
Bristol Rovers v Kidderminster Harriers
Bristol City v Peterborough United
Woking v Leyton Orient
Brentford v Milton Keynes Dons
Swansea City v Luton Town
Barnet v Stevenage Borough
Aldershot Town v Wycombe Wanderers
Shrewsbury Town v Bournemouth
Torquay United v Yeovil
Byes to round two: Swindon & Northampton
Northern Section...
Bradford City v Accrington Stanley
Port Vale v Barnsley
York City v Blackpool
Mansfield Town v Darlington
Macclesfield Town v Chesterfield
Sheffield Wednesday v Chester City
Stockport County v Bury
Rochdale v Scarborough
Lincoln City v Doncaster Rovers
Hereford United v Scunthorpe United
Hartlepool United v Hull City
Huddersfield Town v Morecambe
Notts County v Wrexham
Carlisle United v Grimsby Town
Byes to round two: Oldham & Tranmere Rovers
Ties to be played week commencing 27 September.